camp glosensor 22f vector Search Results


camp glosensor 22f vector  (Promega)
90
Promega camp glosensor 22f vector
Camp Glosensor 22f Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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camp glosensor 22f vector - by Bioz Stars, 2026-04
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mammalian expression plasmid pcdna5 glosensor 22f  (Promega)
90
Promega mammalian expression plasmid pcdna5 glosensor 22f
Mammalian Expression Plasmid Pcdna5 Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mammalian expression plasmid pcdna5 glosensor 22f - by Bioz Stars, 2026-04
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pglosensor-22f camp plasmid e2301  (Promega)
90
Promega pglosensor-22f camp plasmid e2301
Pglosensor 22f Camp Plasmid E2301, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f  (Promega)
90
Promega glosensor-22f
Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
glosensor-22f - by Bioz Stars, 2026-04
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glosensor-22f camp  (Promega)
90
Promega glosensor-22f camp
Glosensor 22f Camp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f camp - by Bioz Stars, 2026-04
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fugene hd  (Promega)
90
Promega fugene hd
Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fugene hd - by Bioz Stars, 2026-04
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glosensor-22f camp gene  (Promega)
90
Promega glosensor-22f camp gene
Glosensor 22f Camp Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor-22f camp gene - by Bioz Stars, 2026-04
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glosensor luciferase-based assay  (Promega)
90
Promega glosensor luciferase-based assay
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Glosensor Luciferase Based Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
glosensor luciferase-based assay - by Bioz Stars, 2026-04
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glosensor tm assay  (Promega)
90
Promega glosensor tm assay
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Glosensor Tm Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glosensor tm assay - by Bioz Stars, 2026-04
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luciferase-based intracellular camp probe glosensor 22 f  (Promega)
90
Promega luciferase-based intracellular camp probe glosensor 22 f
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Luciferase Based Intracellular Camp Probe Glosensor 22 F, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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copn5l2 expression plasmid  (Promega)
90
Promega copn5l2 expression plasmid
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Copn5l2 Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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copn5l2 expression plasmid - by Bioz Stars, 2026-04
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pebmulti-neo vector  (FUJIFILM)
90
FUJIFILM pebmulti-neo vector
Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a <t>GloSensor</t> assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.
Pebmulti Neo Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pebmulti-neo vector - by Bioz Stars, 2026-04
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Image Search Results


Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a GloSensor assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Preclinical Testing of Nalfurafine as an Opioid-sparing Adjuvant that Potentiates Analgesia by the Mu Opioid Receptor-targeting Agonist Morphine

doi: 10.1124/jpet.118.255661

Figure Lengend Snippet: Nalfurafine is a potent G protein-biased KOR agonist. Comparison of G protein and β-arrestin signaling outcomes with a variety of KOR ligands (at indicated concentrations) compared with the reference standard U50,488. (A) G protein signaling was assessed with a GloSensor assay of cyclic AMP inhibition. (B) β-arrestin recruitment to the activated KOR was assessed via the Tango assay. (C and D) Results from GloSensor and Tango assays used to calculate bias factors for each compound from observed maximal efficacy (Emax or “Max”) and potency (EC50) values. ∆∆log(Max/EC50) values, with their 95% confidence intervals (CIs), are plotted in (D) for each compound to indicate relative (to U50,488) bias toward G protein signaling. All compounds were tested in triplicate alongside the reference standard U50,488. N ≥ 3 for all compounds.

Article Snippet: Quantification of G protein signaling-dependent changes in intracellular cyclic AMP (cAMP) levels was conducted using a GloSensor luciferase-based assay in HEK293T cells transiently transfected with 5 μ g of KOR expression vector DNA (3×HA-hKOR; www.cDNA.org ) and 5 μ g of pGloSensor-22F cAMP biosensor expression vector DNA (Promega, Madison, WI).

Techniques: Comparison, Inhibition